HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans

• Authors: JUŘICA Jan; ZENDULKA Ondřej; TOMANDL Josef
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Medicine
• Description: The mehod for simple and reliable determination of caffein and paraxanthine in human saliva was developed. After simple, liquid-liquid extraction into organic solvents, sample were separated on Kinetex PFP column and detected at 268 nm. Mobile phase (0.05% acetic acid: methanol, 75:25) was eluted isocratically at flow rate of 0.55 ml/min. All analytes wre eluted within 13 min.

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