Publication details

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

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Authors

BABULA Petr VODIČKA Ondřej ADAM Vojtěch KUMMEROVÁ Marie HAVEL Ladislav HOŠEK Jan PROVAZNÍK Ivo SKUTKOVÁ Helena BEKLOVÁ Miroslava KIZEK René

Year of publication 2012
Type Article in Periodical
Magazine / Source Environmental and Experimental Botany
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.envexpbot.2011.12.024
Field Physiology
Keywords fluoranthene; BY-2 cells; viability; programmed cell death; fluoranthene accumulation
Description Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000 uM, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.
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