Publication details

Experimental treatment of deep subchondral articular surface defect of the femur

Authors

PLÁNKA Ladislav SRNEC Robert STARÝ David VOJTOVÁ Lucy GÁL Petr KLÍMA Jiří NEČAS Alois

Year of publication 2012
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description Articular cartilage is a tissue with very low regenration ability, therefore the new treatment methods of injuries in this location are constantly sought. Improved healing shows the leasions with current violation of the subchondral bone. Therefore, many treatment methods purposefully violate the subchondral bone, and allowing travel progenitor cells from bone marrow into the defect and ensure at least partial healing (microfracture, forrage). Method This study describes the results of experimental treatment of deep subchondral articular surface defect of the femur (the OCD leason is simulated) with chitosan nanofibers scaffold transplantation staffed mesenchymal stem cells and chondrocytes mixture. Experimental animals were twenty young miniature pigs, the frequency of monitoring 20 weeks. After this period knee arthroscopy evaluation was performed and after the protocol culling of the animal followed the histolgical examination of the healed lesions (O'Driscoll score, Collagen II type, Imunofluorescence - CMDiI). The control group was the same defect created by the stress area of the articular surface of the distal epiphysis of the femur on the other leg, left without treatment. Results Average O'Driscoll score of experimental group A was 15,7 +/- 2,8, in comparison with control group B - O'Driscoll score in average was 4,8 +/- 3,1. In group A higher positivity of Collagen II type (85%) than in group B (25%) was observed as well as in Imunofluorescence (A 80%, B 0%)