Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment

• Authors: SEDLÁČEK Ivo; HOLOCHOVÁ Pavla; MAŠLAŇOVÁ Ivana; KOSINA Marcel; SPRÖER Cathrin; BRYNDOVÁ Hana; VANDAMME Peter; RUDOLF Ivo; HUBÁLEK Zdeněk; ŠVEC Pavel
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: International Journal of systematic and Evolutionary Microbiology
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1099/ijs.0.041152-0
• Field: Microbiology, virology
• Keywords: Enterococcus; taxonomy; description
• Description: A set of 25 urease producing, yellow pigmented enterococci was isolated from environmental sources and studied by using a polyphasic taxonomic approach. The phenotypic classification divided the isolates into two groups and both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all validly named Enterococcus spp. and were placed in the Enterococcus faecalis species group. DNA-DNA hybridisation experiments, pheS and rpoA genes sequencing, and whole-cell protein electrophoresis provided conclusive evidence for the classification of both phena as novel Enterococcus species for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629T = LMG 26676T = CCUG 48799T), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630T = LMG 26678T = CCUG 61593T), inhabiting water, insects (mosquitoes) and plants are proposed

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