Publication details

Polyphasic evaluation of Limnoraphis robusta, a water-bloom forming cyanobacterium from Lake Atitlan, Guatemala, with a description of Limnoraphis gen. nov.

Authors

KOMÁREK Jiří ZAPOMĚLOVÁ Eliška ŠMARDA Jan KOPECKÝ Jiří REJMÁNKOVÁ Eliška WOODHOUSE Jason NEILAN Brett A. KOMÁRKOVÁ Jaroslava

Type Article in Periodical
Magazine / Source Fottea
MU Faculty or unit

Faculty of Medicine

Citation
Web http://fottea.czechphycology.cz/_contents/F13-1-2013-04.pdf
Field Botany
Keywords carotenoids; ecology; Guatemala; Lake Atitlan; Limnoraphis; planktic Lyngbya; taxonomy; ultrastructure; water-blooms
Description A tropical planktic filamentous cyanobacterium, tentatively identified as Lyngbya robusta, recently increased in abundance in Lake Atitlan, Guatemala, and since 2008 annual water-blooms occurred. This was one from the first known cases of L. robusta water-blooms worldwide. A polyphasic evaluation of L. robusta using 16S rRNA gene sequencing, cytomorphological markers, and ecological characteristics was made. This species had several unique features. It produced aerotopes that were irregularly spaced in cells; cyanotoxins were not found, and it fixed nitrogen in spite of the lack of heterocytes. It contained a high amount of carotenoids, which caused an unusual brown color of the macroscopic scum on the water level. Molecular phylogenetic analyses using the 16S rRNA gene showed that L. robusta, together with few other planktic species, formed a clade, separated from typical Lyngbya species. The main diacritical markers of this clade were the planktic type of life and formation of gas vesicles in cells. Based upon molecular, morphological and ecological data, a new genus Limnoraphis was proposed with four species.