Publication details

Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood

Authors

KILLER Jiří DUBNÁ Soňa SEDLÁČEK Ivo ŠVEC Pavel

Year of publication 2014
Type Article in Periodical
Magazine / Source International Journal of Systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web http://ijs.sgmjournals.org/content/64/Pt_1/152.abstract?sid=bc11a653-d638-446b-b762-9ec0c3cdb393
Doi http://dx.doi.org/10.1099/ijs.0.053033-0
Field Microbiology, virology
Keywords Lactobacillus apis sp. nov.; Apis mellifera; taxonomy
Description A taxonomic study was performed on Gram-stain-positive, catalase-negative and regular rod-shaped bacterial strains R4BT and R4C, isolated from the stomachs of honeybees. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strains was within the genus Lactobacillus; the highest sequence similarity to R4BT was shown by Lactobacillus acidophilus BCRC 10695T. Lower sequence similarities were found to other obligately homofermentative lactobacilli. A PCR–DGGE method could detect the sequence of the 16S rRNA gene of strain R4BT at different developmental stages of honeybees occurring in two different locations in the Czech Republic. The distinctiveness of the strains from other lactobacilli was also confirmed by analysis of sequences of other phylogenetic markers applicable to the taxonomy of the genus Lactobacillus, ribotyping and rep-PCR analysis. The DNA G+C content of strain R4BT was 41.3% mol. The major polar lipids of strain R4BT were glycolipids, lipids and phospholipids. Phenotypic and phylogenetic characteristics also confirmed the independent status of the strains at the species level. Interestingly, strain R4BT was able to inhibit growth in vitro of Paenibacillus larvae subsp. larvae (causal agent of American foulbrood in honeybees) and Melissococcus plutonius (causal agent of European foulbrood). The name Lactobacillus apis sp. nov. is proposed for this novel taxon.
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