Publication details

Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

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Authors

DUBOVÝ Petr KLUSÁKOVÁ Ilona HRADILOVÁ SVÍŽENSKÁ Ivana

Year of publication 2014
Type Article in Periodical
Magazine / Source BioMed Research International
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.hindawi.com/journals/bmri/2014/691041/
Doi http://dx.doi.org/10.1155/2014/691041
Field Neurology, neurosurgery, neurosciences
Keywords activated Schwann cell; growing axons; reinnervation; inflammatory mediators
Description Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.
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