Publication details
Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | ENGINEERING IN LIFE SCIENCES |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1002/elsc.201300165 |
| Field | Biotechnology |
| Keywords | Carbon dioxide; Exponential phase; Growth optimization; Light; Temperature |
| Attached files | |
| Description | We characterized the photoautotrophic growth of glucose-tolerant Synechocystis sp. PCC 6803 in a flat-panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h(-1), which corresponds to a doubling time of 5.13 ha growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220-360 mol(photons)m(-2)s(-1), and we did not observe any photoinhibition up to 660 mol(photons)m(-2)s(-1). Synechocystis was able to grow under red light only; however, photons of wavelengths 405-585 and 670-700 nm further improved its growth. Optimal growth temperature was 35 degrees C. Below 32 degrees C, the growth rates decreased linearly with temperature coefficient (Q(10)) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculationculture exponential growthis often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time-resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure. |