Molecular and ultrastructural characterisation of Balantidium coli parasitizing orang-utans Pongo abelii and Pongo pygmaeus in Indonesia: Possible medicinal use of plants against balantidiasis.
|Year of publication||2014|
|Type||Appeared in Conference without Proceedings|
|MU Faculty or unit|
|Description||During our parasitological monitoring of wild and semi-wild orang-utans, throughout Sumatra and Kalimantan, 16 different parasitic species were identified. Balantidium coli that could cause severe morbidity and fatalities had the highest prevalence observed. For molecular characterization, the region of ribosomal DNA gene cluster (ITS1, 5.8S, ITS2) was amplified, sequenced and compared. The NJ/MP/ML phylogenetic analysis included comparison of data for order Vestibuliferida, families Balantidiidae (genera Balantidium, Neobalantidium) and Pycnotrichidae (genus Buxtonella). Data from the relative genus Troglodytella (Troglodytellidae, Entodiniomorphida) were used as an out-group. Our samples clustered in separate clades, corresponding to the different host origins. The progress in the identification of biologically active plant extracts with potential anti-parasitic properties (treating of balantidiasis) and their applications was also discussed.|