Publication details

Cysteine peptidase inhibitors of Trichinella spiralis



Year of publication 2015
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Trichinella spriralis is well known causative agent of human disease trichinellosis. Individuals of this nematode parasite produce various types of cysteine peptidase inhibitors (cystatins) in order to regulate the function of cysteine peptidases and modulate the immune response of the host organism. Therefore we aim to reveal the differences in production of cystatin by different T. spiralis developmental stages. Three types of cystatins were identified in the genome of T. spiralis – cystatin B, onchocystatin and multi cystatin domain protein. ICR and SCID mice were experimentally infected per os with infective muscle stage larvae (ML) of T. spiralis. Preadults (Ad3), adults (Ad5), new born larvae (NBL) and muscle stage (ML) larvae of T. spiralis were isolated from the intestinal and muscle tissue at various time post infection. Excretory/secretory products of all developmental stages were isolated and screened for the presence of cystatins using mass spectrometry. The cDNA was reversely transcribed from RNA of all stages and genes for cystatins were amplified using specific primers. Production of three types of cystatins by all studied stages was analyzed and evaluated.
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