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Nové směry ve studiu biomolekulárních interakcí pomocí kapilární elektroforézy

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Title in English New Directions in the Study of Biomolecular Interactions by Capillary Electrophoresis


Year of publication 2016
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Field Biochemistry
Keywords capillary electrophoresis; biomolecular interactions; frontal analysis; affinity capillary electrophoresis; Hummel Dreyer methods; vacancy peak; vacancy affinity capillary electrophoresis
Description This review provides overview of electrophoretic techniques used for determination of binding parameters such as binding constant and number of binding sites. Various approaches based on CE are described. It focuses on the experimental arrangement of methods and on possible results obtained by them. Beside the advantages of CE for determination of binding parameters are emphasized and compare as well.
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