Publication details

Statistical comparison of excystation methods in Cryptosporidium parvum oocysts

Investor logo

PECKOVÁ Radka STUART Peter Daniel SAK Bohumil KVĚTOŇOVÁ Dana KVAČ Martin FOITOVÁ Ivona

Year of publication 2016
Type Article in Periodical
Magazine / Source Veterinary Parasitology
MU Faculty or unit

Faculty of Science

Field Zoology
Keywords Cryptosporidium parvum; excystation methods; in vitro cultivation; sodium hypochlorite; trypsin
Attached files
Description The objectives of our study were to compare different excystation protocols of the oocysts of C. parvum to find the most effective one. Excystation is an essential process for successful in vitro assays and using the right method can save time and material to a considerable extent. The protocols that we tested differed in chemical pre-incubation steps, excystation media or time of incubation. Those pre-incubation steps which included sodium hypochlorite were found to increase the excystation ratio and methods using this step are therefore the most effective.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info