Publication details

Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin

Authors

ROUDNICKÝ Pavel VOREL Jiří ILGOVÁ Jana MIKEŠ Libor JEDLIČKOVÁ Lucie DALTON John DVOŘÁK Jan ZDRÁHAL Zbyněk POTĚŠIL David JANDA Lubomír NOREK Adam GELNAR Milan KAŠNÝ Martin

Year of publication 2016
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
Description Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.
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