Publication details

Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors

Authors

MAKHNEVA Ekaterina FARKA Zdeněk OBRUSNÍK Adam MICHLÍČEK Miroslav SKLÁDAL Petr ZAJÍČKOVÁ Lenka

Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description Biosensors have been extensively developed and applied for biomedical and environmental studies. Quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors appeared to be very promising tools regarding the sensitive determination of various antigens and bacteria. For the development of effective biosensors, the immobilization of the biorecognition biomolecules onto the sensor surface is always required. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity allowing the formation of the covalent linkages between biomolecules and a surface. For most applications, a sufficiently high surface concentration of functional groups as well as the layer stability in different buffers is required. In our work, stable carboxyl-rich plasma polymers (COOH PPs) were deposited using plasma co-polymerization of maleic anhydride and acetylene at atmospheric pressure using dielectric barrier discharge (DBD). Concentrations of carboxyl groups were determined by well-known derivatization with trifluoroethanol (TFE). For the immunosensors development 20 nm thick COOH PPs were deposited onto the gold surfaces of QCM electrodes and SPR chips. Then the monoclonal antibody AL-01, specific to human serum albumin (HSA) was immobilized using EDC/NHS activation of COOH groups in the polymer. Performance of QCM and SPR immunosensors was evaluated by the immunoassay flow test.
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