Publication details
Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells
| Authors | |
|---|---|
| Year of publication | 2017 |
| Type | Article in Periodical |
| Magazine / Source | CANCER IMMUNOLOGY IMMUNOTHERAPY |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1007/s00262-016-1932-4 |
| Field | Oncology and hematology |
| Keywords | Bacterial ghosts; Dendritic cells; T cell stimulation; Cancer immunotherapy; Natural adjuvant; Vaccine |
| Description | The natural adjuvant properties of bacterial ghosts (BGs) lie within the presence of intact pathogen-associated molecular patterns on their surface. BGs can improve the direct delivery, natural processing and presentation of target antigens within dendritic cells (DCs). Moreover, sensitization of human DCs by cancer cell lysate (oncolysate)-loaded BGs in the presence of IFN-alpha and GM-CSF enhanced DC maturation as indicated by an increased expression of maturation markers and costimulatory molecules, higher production of IL-12p70 and stimulation of significantly increased proliferation of both autologous CD4(+) and CD8(+) T cells compared to DCs matured in the presence of purified lipopolysaccharide. The induced T cells efficiently recognized oncolysate-derived tumor-associated antigens expressed by cancer cells used for the production of oncolysate. Our optimized one-step simultaneous antigen delivery and DC maturation-inducing method emerges as a promising tool for the development and implementation of next-generation cellular cancer immunotherapies. |