Publication details

On-capillary derivatizations of amino acids in capillary electrophoresis using naphthalene-2,3-dicarboxaldehyde/sodium cyanide

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CELÁ Andrea MÁDR Aleš GLATZ Zdeněk

Year of publication 2017
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Description Amino acids are metabolites of great importance to living organisms. Their actual levels in biological fluids can reveal complex physiological state of an organism, thus the determination of amino acids can be used for diagnostic purposes. Unfortunatelly, they exhibit low or no native fluorescence, thus the derivatization is essential for their sensitive determination by fluorescence detection. Capillary electrophoresis offers derivatization within the capillary, where the inner space of the capillary is used at first for derivatization and then for separation. This approach can be fully automated and requires only minimum volume of sample and other reagents. Optimization of two methods for derivatization of amino acids with naphthalene 2,3 dicarboxaldehyde/ sodium cyanide, differing in mixing strategy of the reactants, was performed. This contribution deals with comparison of the methods based on mixing by transverse diffusion of laminar flow profiles (TDLFP) and by electrophoretically mediated microanalysis (EMMA) in terms of efficiency of the mixing, sensitivity, repeatability and applicability to various biological samples.
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