Publication details

Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

Authors

ROUDNICKÝ Pavel LOJA Tomáš POTĚŠIL David ZDRÁHAL Zbyněk GELNAR Milan KAŠNÝ Martin

Year of publication 2018
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
Description Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.
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