Publication details

Intrinsic atomic interaction at molecular proximal vicinity infer cellular biocompatibility of antibacterial nanopepper


JHA Ealisha PANDA Pritam Kumar PATEL Paritosh KUMARI Puja MOHANTY Swabhiman PARASHAR S.K.S. AHUJA Rajeev VERMA Suresh K. SUAR Mrutyunjay

Year of publication 2021
Type Article in Periodical
Magazine / Source NANOMEDICINE
MU Faculty or unit

Faculty of Science

Keywords antibacterial; black pepper; caspase; cytotoxicity; HCT116 cells; piperine; Sod1
Description Aim: Fabrication of nanopepper (NP) for antibacterial application and elucidation of its molecular and cellular biocompatibility. Materials & methods: Synthesis of NP was achieved using a high-energy ball milling method. Following characterization, its antibacterial activity and cellular and molecular biocompatibility were evaluated in vitro by experimental and computational approaches. Results: A total of 15 h of milling pepper produced NP with a size of 44 +/- 12 nm and zeta potential of -22 +/- 12 mV. Bulk pepper and NP showed antibacterial activity and an LC50 of 1.9 mu M and 2.1 mu M in HCT116 colon cells. Components of pepper, piperine and beta-caryophyllene were found to interact with superoxide dismutase [Cu-Zn] and apoptotic protease-activating factor-1-caspase-9 through different amino acids via H-bonds. Conclusion: NP exhibits significant antibacterial activity with cellular biocompatibility due to intrinsic atomic interaction.

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