Publication details
Instrumental analytical tools for mycobacteria characterisation
| Authors | |
|---|---|
| Year of publication | 2021 |
| Type | Article in Periodical |
| Magazine / Source | Czech Journal of Food Sciences |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.agriculturejournals.cz/web/cjfs.htm?type=article&id=69_2021-CJFS |
| Doi | http://dx.doi.org/10.17221/69/2021-CJFS |
| Keywords | flow cytometry; MALDI-TOF spectrometry; Raman spectroscopy; infrared spectroscopy; biosensors |
| Attached files | |
| Description | Mycobacteria in drinking water and in the water of swimming pools, whirlpools, hydrotherapy facilities and aquaria contribute significantly to human exposure to triggers of immune regulated chronic inflammatory and auto immune diseases. Technological elements of water distribution systems, especially their inner surface, taps, shower heads and blind spots where sediments settle, affect the number of mycobacteria in the water. The review presents the possibilities of using analytical instruments for rapid determination of mycobacteria and for their typing as an alternative to classical culture and a method of monitoring specific nucleic acid sequences by polymerase chain reaction (PCR). Information about the use of flow cytometry (FCM), matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) spectrometry, Raman and infrared (IR) spectroscopy and biosensors are presented. |