Publication details

Instrumental analytical tools for mycobacteria characterisation


OŽANA Veronika HRUŠKA Karel

Year of publication 2021
Type Article in Periodical
Magazine / Source Czech Journal of Food Sciences
Keywords flow cytometry; MALDI-TOF spectrometry; Raman spectroscopy; infrared spectroscopy; biosensors
Attached files
Description Mycobacteria in drinking water and in the water of swimming pools, whirlpools, hydrotherapy facilities and aquaria contribute significantly to human exposure to triggers of immune regulated chronic inflammatory and auto immune diseases. Technological elements of water distribution systems, especially their inner surface, taps, shower heads and blind spots where sediments settle, affect the number of mycobacteria in the water. The review presents the possibilities of using analytical instruments for rapid determination of mycobacteria and for their typing as an alternative to classical culture and a method of monitoring specific nucleic acid sequences by polymerase chain reaction (PCR). Information about the use of flow cytometry (FCM), matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) spectrometry, Raman and infrared (IR) spectroscopy and biosensors are presented.

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