Publication details
Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry
| Authors | |
|---|---|
| Year of publication | 2022 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Electroanalytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.sciencedirect.com/science/article/pii/S157266572200889X?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.jelechem.2022.116897 |
| Keywords | TNF-? Immunoassay; Metal organic framework; Label-free electrochemical immunosensor; Gold nanoparticles; Etanercept |
| Description | A new simple label-free electrochemical immunosensor was designed for sensitive and selective determination of tumor necrosis factor-alpha (TNF-?), through gold nanoparticles (GNPs) incorporated copper benzene tricarboxylate metal organic frameworks (HKUST-1) modified gold electrode, where TNF-? specific monoclonal antibody was bonded to the electrode surface through shaped self-assembled monolayer (SAM) of mercapto compounds. The fabrication steps were monitored by field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized experimental conditions, fast Fourier transform admittance voltammetry (FFTADV) used for TNF-? measurement. A wide linear range of current response towards the TNF-? concentration from 0.0005 to 50 ng/mL and LOD value of 0.15 pg/mL were achieved using FFTADV. The described immunosensor had adequate sensitivity and selectivity towards direct determination of TNF-? in complex biological matrix. |