Publication details
Determination of optimal conditions for detection of acute myeloid leukemia t(18;21) and t(15;17) translocations using RT-PCR
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Neoplasma |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | RT-PCR; AML; translocation |
| Description | Prognosis of patients with acute myeloid leukemia (AML) is not satisfactory. The aim of the study is to improve diagnosis of MAL in cases characterized by specific chromosomal translocations t(18;21) and t(15;17) using RT-PCR. To determine optimal conditions for detection of both translocations we performed a detail study of experimental onditions for RT-PCR on human cell lines NB-4 and KASUMI-1. The results proved to be valid for clinical diagnstics of the translocations in blood and/or bone marrow samples of AML patients. |
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