Publication details
Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defence mechanisms
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Keywords | Bombyx mori; hemocytes; ROM |
| Description | The aim of this study was to investigate whether hemocytes of Bombyx mori (Lepidoptera) larvae can produce reactive oxygen species (ROS) as a tool for the oxidative killing of invading pathogens. The production of ROS measured as a luminol- and lucigenin-enhanced chemiluminescence of unstimulated or stimulated (zymosan particles, phorbol myristate acetate, calcium ionophore, rice starch or Xenorhabdus nematophila) hemolymph was not detectable. To eliminate the influence of the antioxidative potential of hemolymph plasma (extremely high when measured by the chemiluminescence method), isolated hemocytes were also tested. However, spontaneous as well as the activated generation of ROS by isolated hemocytes remained at the background level. No ROS production by isolated hemocytes was observed through spectrophotometric (NBT test, cytochrome c assay) and fluorometric (using dihydrorhodamine and hydroethidine probes) analyses. To conclude, none of the several experimental approaches used in this study indicated the production of ROS by hemocytes of Bombyx mori larvae as a part of their immune response. |