Publication details

Mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture

Authors

KOPECKÝ Jan BAUDYŠOVÁ Marie PAVELKA Stanislav ZANOTTI Francesco JANÍKOVÁ Dagmar HOUŠTĚK Josef

Year of publication 1991
Type Article in Periodical
Magazine / Source Journal of Cellular Biochemistry [IF=2.778 (1990)]
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Biogenesis; brown adipose tissue; thermogenic mitochondria; uncoupling protein
Description Differentiation of precursor cells isolated from mouse brown adipose tissue was studied in cell culture, in order to characterize the biogenesis of unique thermogenic mitochondria. Synthesis of mitochondrial uncoupling protein (UCP), F1-ATPase, and cytochrome oxidase was examined by L-[35S]methionine labeling and immunoblotting. In these cells, it was possible, by using norepinephrine, to induce specifically the synthesis of the UCP but not of F1-ATPase or cytochrome oxidase.

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