Publication details
Identifikace nádorově-specifických T lymfocytů na základě produkce interferonu gama u mnohočetného myelomu
| Title in English | Identification of tumor-specific cells based on interferongamma production in multiple myeloma |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| Keywords | Multiple myeloma; immunotherapy; interferon gamma; T cell |
| Description | Backgrounds:Multiple myeloma is a hematological disease cause by clonal proliferation of a malignant plasma cell clone. Its incidence is 4 in 100 000. Median survival is 4-5 yers. Besides standard therapy including high-dose chemotherapy new approaches such as immunotherapy are taking place.Design and subjects: Identification of myeloma-specific T cells from healthy blood donors has been tested in an in vitro study based on T cell activation, separation and expansion. Methods and results: Myeloma cell line ARH 77 has been irradiated and used as apoptotic bodies to stimulate allogeneic mononuclear cells from peripheral blood. Activated myeloma-specific T cells produce interferon gamma, can be captured by immunomagnetic beads and further expanded in vitro up to 400x10.6 T cells within 4 weeks.Conclusion: This study demonstrates feasibility of identification and separation of tumor-specific T cells that can be expanded in vitro to numbers used in clinical application. |