Publication details

Genotype variability of the enterotoxin H-positive <I>Staphylococcus aureus</I> strains isolated from patients and foods in the Czech Republic



Year of publication 2008
Type Article in Proceedings
Conference 13th International Symposium on Staphylococci and Staphylococcal Infections
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Keywords Staphylococcus aureus; enterotoxin H
Description Objective: Staphylococcal enterotoxin H (SEH) is a member of the extracellular toxin family secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Genetic properties of the 32 SEH-positive S. aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were studied. One strain, detected in feta cheese, was implicated in a case of SFP. Methods: Genotyping of strains was performed by PCR detection of the genes for the enterotoxins SEA-SEJ, spa gene polymorphism analysis, PFGE profiling, ERIC2-PCR fingerprinting and prophage carriage. Results: Sixteen of 28 strains carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec genes and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of various genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one isolate from raw meat and two strains from pizza. Conclusions: Our results show that the SEH-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
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