The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

• Authors: BLÁHOVÁ Lucie; ORAVEC Michal; MARŠÁLEK Blahoslav; ŠEJNOHOVÁ Lenka; ŠIMEK Zdeněk; BLÁHA Luděk
• Year of publication: 2009
• Type: Article in Periodical
• Magazine / Source: Toxicon
• MU Faculty or unit: Faculty of Science
• Field: Environment influence on health
• Keywords: cyanotoxin;cylindrospermopsin;methods of detection
• Description: We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.

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