Chromatographic (RP-HPLC) analysis of cholesterol and selected phytosterols

• Authors: HÉGROVÁ Blanka; YEUNG Edward S.; PREISLER Jan
• Year of publication: 2009
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: Phytosterols (plant sterols) are secondary metabolites abundant in all vegetable foods; they resemble cholesterol in vertebrates in terms of both function and structure. Current methods for the analysis of cholesterol as well as other sterols and lipid classes include TLC, SPE, GC, HPLC and SFC. RP-HPLC conditions include mild column temperature, non-destructive detection and less volatile polar organic solvents in water. In this study, chromatographic separation of sterols was optimized for their fraction collection and subsequent MALDI MS determination using silver nanoparticles as matrix.

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