SPECTROSCOPIC STUDY OF PROTONATION EQULIBRIA OF SHORT OLIGONUCLEOTIDES CONTAINING CYTOSIN

• Authors: HOLUBOVÁ Sylvie; LUBAL Přemysl; TRNKOVÁ Libuše
• Year of publication: 2010
• Type: Article in Proceedings
• Conference: X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: oligonucleotides; cytosine; equilibria; spectroscopy
• Description: This paper deals with the spectroscopic study of protonation of short deoxyoligonucleotides (ODNs) containing cytosine. The measurements were performed at constant temperature and ionic strength. The influence of the number of cytosine in the ODNs chain on protonation was studied. The pKa values were calculated using the software OPIUM. Constants pKa determined at several temperatures provided the possibility to calculate the thermodynamic parameters such as enthalpy and entropy.

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