Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris.
|Year of publication||2012|
|Type||Article in Periodical|
|Magazine / Source||PLoS Biology|
|MU Faculty or unit|
|Keywords||Y-CHROMOSOME; DNA-SEQUENCES; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; REPETITIVE DNA; SIZE; EVOLUTION; ACCUMULATION; SYSTEM; GENE|
|Description||We analyzed differences in the number and distribution of major repetitive DNA elements in two closely related species, Silene latifolia and S. vulgaris. Both species are diploid and possess the same chromosome number (2n = 24), but differ in their genome size and mode of reproduction. The dioecious S. latifolia (1C = 2.70 pg DNA) possesses sex chromosomes and its genome is 2.5x larger than that of the gynodioecious S. vulgaris (1C = 1.13 pg DNA), which does not possess sex chromosomes. We discovered that the genome of S. latifolia is larger mainly due to the expansion of Ogre retrotransposons. Surprisingly, the centromeric STAR-C and TR1 tandem repeats were found to be more abundant in S. vulgaris, the species with the smaller genome. We further examined the distribution of major repetitive sequences in related species in the Caryophyllaceae family. The results of FISH (fluorescence in situ hybridization) on mitotic chromosomes with the Retand element indicate that large rearrangements occurred during the evolution of the Caryophyllaceae family.|