Human genome structure
- Project Identification
- Project Period
- 1/2003 - 12/2007
- Investor / Pogramme / Project type
- Academy of Sciences of the Czech Republic
- MU Faculty or unit
- Faculty of Informatics
- nuclear architecture; chromosome structure; FISH; high-resolution cytometry;
- Cooperating Organization
Institute of Biophysics
One of the main directions of human genome research in the post-genomic eta is the study of its higher-order structure that became the key to understanding of the most important cellular processes such as cell differentiation or transformation. Using high-resolution cytometry developed in our laboratory, nuclear and territorial topography of genetic elements will be systematically investigated in normal and malignant human cell types. The topography and its changel will be correlated with local acetylation of histones, methylation of DNA and expression of gene clusters. In addition, changes of the structure of chromosome terriories or whole human genome in the cell nucleus during differentiation and cancerogenesis will be determined and an attempt will be made to find the basic psrinciples of the epigenetic regulation of gene expression. The practical goal of the project is the use of experimental results and technology developed in our laboratory in diagnostics ant therapy of cancer.
The practical goal of the project is the use of experimental results and technology developed in our laboratory in diagnostics and therapy of cancer.
Total number of publications: 32