Project information

New possibilities of diagnostics of leukemia using DNA-microarrays

Project Identification
Project Period
7/2004 - 12/2006
Investor / Pogramme / Project type
Ministry of Health of the CR
MU Faculty or unit
Faculty of Informatics
DNA microarrays;histone modifications;inhibitors of deacetylases;chromatin structure;leukemia;histon
Cooperating Organization
Institute of Biophysics
University Hospital Brno-Bohunice

Using DNA microarrays, gene expression will be studied in leukemia, in control cell populations and in cell cultures. Changes will be correlated
to epigenetic characteristics of chromatin at the level of its modifications (methylation, acetylation). Structural characteristics of chromatin at sites with altered expression will be studied using fluorescence in situ hybridization (FISH), spectral microscopy and immunoFISH. Drugs with narrow spectrum of genes with altered expression wil be studied. Using ad vanced techniques of gene or protein visualization and top level confocal microscopy, the mechanisms of the induction and progression of leukemia will be investigated with the goal to suggest appropriate markers for diagnostics. We hope that our study of gene expression in parallel to epigenetics will allow us to propose target molecules of effective therapy.


Total number of publications: 19

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