Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress
- Project Identification
- NV19-03-00091
- Project Period
- 5/2019 - 12/2022
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- Ministry of Health Research Programme 2015 - 2022
- MU Faculty or unit
- Central European Institute of Technology
- Cooperating Organization
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University Hospital Brno-Bohunice
- Responsible person Mgr. Veronika Navrkalová, Ph.D.
Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.
Publications
Total number of publications: 45
2023
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Comprehensive NGS testing allows integrated assessment of individual patient prognosis in multiple myeloma.
Year: 2023, type: Conference abstract
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Distinct p53 phosphorylation patterns in chronic lymphocytic leukemia patients are reflected in the activation of circumjacent pathways upon DNA damage
MOLECULAR ONCOLOGY, year: 2023, volume: 17, edition: 1, DOI
2022
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Comparison of DNA and RNA-based approaches for the genetic characterization and stratification of patients with diffuse large B-cell lymphoma
Year: 2022, type: Conference abstract
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Cytogenetics in Chronic Lymphocytic Leukemia: ERIC Perspectives and Recommendations
HemaSphere, year: 2022, volume: 6, edition: 4, DOI
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Dynamics of genomic aberrations in relation to disease activity in untreated patients with chronic lymphocytic leukemia
Year: 2022, type: Conference abstract
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Evolution of TP53 abnormalities during CLL disease course is associated with telomere length changes
BMC Cancer, year: 2022, volume: 22, edition: 1, DOI
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Gene expression analysis on single cell level uncovers the subclonal architecture of chromosomes in a leukemia patient with multiple chromosomal aberrations.
Year: 2022, type: Conference abstract
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Identification of genetic aberrations in multiple myeloma patients with low tumour burden using comprehensive NGS testing.
Year: 2022, type: Conference abstract
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Impact of the Types and Relative Quantities of IGHV Gene Mutations in Predicting Prognosis of Patients With Chronic Lymphocytic Leukemia
Frontiers in Oncology, year: 2022, volume: 12, edition: July 2022, DOI
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In-frame deletions and insertions in the TP53 gene identified in leukemia patients result in p53 protein inactivation.
Year: 2022, type: Conference abstract