Project information
Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress
- Project Identification
- NV19-03-00091
- Project Period
- 5/2019 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Ministry of Health Research Programme 2015 - 2022
- MU Faculty or unit
- Central European Institute of Technology
- Cooperating Organization
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University Hospital Brno-Bohunice
- Responsible person Mgr. Veronika Navrkalová, Ph.D.
Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.
Publications
Total number of publications: 46
2024
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Integrative NGS testing reveals clonal dynamics of adverse genomic defects contributing to a natural progression in treatment-naïve CLL patients
British journal of haematology, year: 2024, volume: 204, edition: 1, DOI
2023
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Comprehensive NGS testing allows integrated assessment of individual patient prognosis in multiple myeloma.
Year: 2023, type: Conference abstract
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Different prognostic impact of recurrent gene mutations in chronic lymphocytic leukemia depending on IGHV gene somatic hypermutation status: a study by ERIC in HARMONY
Leukemia, year: 2023, volume: 37, edition: 2, DOI
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Distinct p53 phosphorylation patterns in chronic lymphocytic leukemia patients are reflected in the activation of circumjacent pathways upon DNA damage
MOLECULAR ONCOLOGY, year: 2023, volume: 17, edition: 1, DOI
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Subclonal architecture of chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL.
Year: 2023, type: Conference abstract
2022
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Comparison of DNA and RNA-based approaches for the genetic characterization and stratification of patients with diffuse large B-cell lymphoma
Year: 2022, type: Conference abstract
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Cytogenetics in Chronic Lymphocytic Leukemia: ERIC Perspectives and Recommendations
HemaSphere, year: 2022, volume: 6, edition: 4, DOI
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Dynamics of genomic aberrations in relation to disease activity in untreated patients with chronic lymphocytic leukemia
Year: 2022, type: Conference abstract
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Evolution of TP53 abnormalities during CLL disease course is associated with telomere length changes
BMC Cancer, year: 2022, volume: 22, edition: 1, DOI
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Gene expression analysis on single cell level uncovers the subclonal architecture of chromosomes in a leukemia patient with multiple chromosomal aberrations.
Year: 2022, type: Conference abstract