In-cell NMR spectroscopy is the only analytical method that enables the structural characterization of proteins and their interactions in living cells at atomic resolution. It has recently emerged as a unique tool for addressing various research questions related to human physiology and drug development. Its applications include target structure validation, drugtarget engagement assessment, and evaluation of drug-target affinity under native-like conditions. However, in-cell NMR spectroscopy in human cells has been limited to asynchronous single-cell(-type) cultures. Unfortunately, these cultures do not reliably recapitulate the key functional properties that cells exhibit when part of a tissue. Additionally, many cell types cannot be maintained in traditional culture conditions but can only be propagated when differentiated in situ from progenitor (pluripotent stem) cells as part of a human tissue-like environment. Reliance on single-cell(-type) cultures represents a major limitation of current in-cell NMR applications in drug development and basic research.

Sustainable Development Goals

Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.