Publication details

Genetický marker RGH1aE2I2 pro rezistenci k původci padlí travního u ječmene

Title in English Genetic marker RGH1aE2I2 for powdery mildew resistance in barley
Authors

ŘEPKOVÁ Jana SOLDÁNOVÁ Martina

Year of publication 2012
MU Faculty or unit

Faculty of Science

Web http://download.springer.com/static/pdf/680/art%253A10.1007%252Fs10681-009-0096-0.pdf?auth66=1362648435_e00056c0c6e061a50e4e4e7bdfa5604a&ext=.pdf
Description DNA marker is developed from the sequence of the RGH1a gene (exon 2 and intron 2) at the Mla locus on chromozomu 1H in barley (Hordeum vulgare). The primer sequences (5`-CTGCTAGTCATCCGTGCAGA-3`, 5`-ACGATTTGTGTGAGCACGAG-`3) and amplification cycle were designed for detection of target sequence. Amplified DNA fragment 506 bp is detected by gel electrophoresis. Functional sample was made by verifying of properties.
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