Publication details

Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in the Czech Republic

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Title in English Antimikrobiální rezistence kmenů Listeria monocytogenes izolovaných od lidí v České republice


Year of publication 2013
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Listeria monocytogenes is generally susceptible to a wide range of antimicrobials. However resistant or multidrug-resistant strains of L. monocytogens isolated from human, food or environment were reported, too. The aim of this study was to observe whether the susceptibility of L. monocytogenes of human origin has changed in the period from 2001 to 2012 in the Czech Republic. In total, 169 human strains isolated from different regions of the Czech Republic were tested. Strains originated from blood cultures (83), cerebrospinal fluid (28) and other material (58) like newborns, liver, etc. Phenotypic resistance testing has been performed by the disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI, 2006). Following antimicrobial agents were tested: ampicillin, penicillin, erythromycin, gentamicin, trimethoprim, vancomycin, tetracyclin and chloramfenicol. The zone diametr breakpoints for Staphylococcus spp. were applied with the exception for penicillin and ampicillin for these agents criteria for Enterococcus spp. were applied (CLSI, 2006). None of the tested strains revealed resistance to the tested panel of antimicrobial agents, all strains showed good sensitivity to drugs used for treatment of listeria infections. Since the year 2012 EUCAST has recommended clinical MICs and zone diameters of L. monocytogenes for antibiotics use in human therapy. This fact can help to compare results of antibiotic susceptibility of L. monocytogenes between various studies on international level. Our results indicate very good susceptibility of human strains of L. monocytogenes to antimicrobials used in the treatment of human listeriosis in the Czech Republic during the monitoring period.
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