Publication details

Minimum aeration in Acidithiobacillus ferrooxidans cultures required to maintain substrate oxidation without oxygen limitation

Authors

MANDL Martin PAKOSTOVÁ Eva POSKEROVÁ Lenka

Year of publication 2013
Type Article in Proceedings
Conference 20th International Biohydrometallurgy Symposium, IBS 2013
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.4028/www.scientific.net/AMR.825.414
Field Mining industry incl. coal mining and processing
Keywords Acidithiobacillus ferrooxidans; aeration; iron-oxidising bacteria; oxygen limitation; sulphur-oxidizing bacteria; volumetric oxygen transfer coefficient
Description The volumetric oxygen transfer coefficient (kLa) was used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation in bioleaching cultures of Acidithiobacillus ferrooxidans. The Michaelis constants for oxygen were 1.07 and 0.71 umol O2/l for the oxidation of ferrous iron and elemental sulphur, respectively. The critical oxygen concentration, below which oxygen limitation occurred, was determined to be 6.250 and 3.125 umol O2/l for the oxidation of ferrous iron and elemental sulphur, respectively. The (kLa)crit values required to maintain oxygen-unlimited substrate oxidation for ferrous iron and elemental sulphur were 7.70 and 4.88/h, respectively.

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