Publication details

Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea).

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Year of publication 2014
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Blood-feeding monogenean Eudiplozoon nipponicum parasitizing the gills of common carp (Cyprinus carpio) is widely distributed Euroasian ectoparasite from Diplozooidae family. The foregoing research has been oriented toward understanding the morphology and ecology of particular species from this taxonomic group. Currently only a little is known about the mechanisms of host-parasite interactions associated with the functional molecules produced by this parasites. Among these molecules appear also the cystatins - inhibitors of cysteine peptidases. These were previously proven to play a substantial role in manipulation of the host immune response by various parasitic species and thus maximizing the parasite success. By revealing the function of cystatin of Eudiplozoon nipponicum we would like to fill the gap in the present knowledge concerning the molecules from diplozoid parasites. By using the data based on Eudiplozoon nipponicum transcriptome we identified cystatine gene sequence homologous to cystatins of other related platyhelminth species. We designed specific primers, amplified, cloned and sequenced the gene coding cystatin. Currently we are optimizing the recombinant protein expression in E. coli expression system (pET19b vector plasmid, E. coli BL21 strain). Recombinant form of E. nipponicum cystatin will be characterized in detail.
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