Publication details

Microsatellites versus SNP polymorphisms, methods of detection and their utilization. Meetings and Abstracts of the Czechoslovak Biological Society. Scripta Medica Facultatis Medicae Universitatis Masarykianae Brunensis – 77 (5-6): 323-324. ISSN: 1211-3395



Year of publication 2004
Type Conference abstract
Description Our main challenge was to use the convenient and most reliable method to find out genetic differences among animal individuals, to assess their influence on the quantitative trait loci and the production traits, or eventually, use them for parentage or individual testing. The progressive recent technologies in nucleotide polymorphism detection take note of need to analyze the big amount of samples in one test tube as quickly as possible. This advantage offer mainly technologies of multiplex PCR and DNA pooling. As a result, we confirmed the convenience of SNaPshot minisequencing usage and we find it very time saving, reliable and efficient in testing of panel of seven genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF3, and MC3R) with reproducible results. This complete system contains everything required for precise and cost-effective SNP analysis and could replace the contemporary methods used in routine testing.

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