Publication details

Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study

Authors

RUDÁ Jana AMCHOVÁ Petra HAVLICKOVA Tereza JERABEK Pavel BABINSKÁ Zuzana KACER Petr SYSLOVA Kamila ŠULCOVÁ Alexandra SUSTKOVA-FISEROVA Magdalena

Year of publication 2015
Type Article in Periodical
Magazine / Source The World Journal of Biological Psychiatry
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.tandfonline.com/doi/full/10.3109/15622975.2015.1077991#abstract
Doi http://dx.doi.org/10.3109/15622975.2015.1077991
Field Pharmacology and pharmaceutical chemistry
Keywords Depression; in vivo microdialysis; methamphetamine; olfactory bulbectomy; rats
Attached files
Description OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, GABA and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
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