Publication details

Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry

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ŠPANOVÁ Alena DRÁB Vladimír TURKOVÁ Kristýna ŠPANO Miroslav BURDYCHOVÁ Radka ŠEDO Ondrej ŠRŮTKOVÁ Dagmar RADA Vojtěch RITTICH Bohuslav

Year of publication 2015
Type Article in Periodical
Magazine / Source European Food Research and Technology
MU Faculty or unit

Central European Institute of Technology

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Field Microbiology, virology
Keywords Lactobacillus; Identification; MALDI-TOF MS; In vitro tests; Probiotic characteristics
Description In the present study, phenotypic and genotypic methods and MALDI-TOF mass spectrometry were applied for the species identification of 29 out of 30 strains of the genus Lactobacillus isolated from faecal samples of 30 breastfed infants. All strains were tested for growth in milk and/or reconstituted skimmed milk supplemented with 0.5 % yeast extract, production of lactic acid, bile salts and low pH tolerance, and for production of beta-glucuronidase. PCR screenings of tyrosine-decarboxylase and histidine-decarboxylase genes were also carried out. Survival of Lactobacillus cells at low pH and their bile tolerance were strain specific. Finally, seven strains of probiotic species were selected for possible use in the dairy industry and for further tests concerning their probiotic characteristics.
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