Publication details

Redox Processes of Guanine Moieties in DNA Heptamers Related to Hydrogen Evolution



Year of publication 2016
Type Article in Periodical
Magazine / Source Electroanalysis
MU Faculty or unit

Faculty of Science

Field Physical chemistry and theoretical chemistry
Keywords Hairpins; NMR of DNA heptamers; cyclic voltammetry; hydrogen evolution reaction; vertex potential; neurodegenerative diseases
Description The paper is focused on the electrochemical investigation of DNA heptamers d(GCXXXGC) with different central triplets of nucleotides (XXX=GAA, AAA, CCC, GGG). On a hanging mercury electrode (HMDE), the reduction peaks of adenine (A) and cytosine (C) moieties are detectable, but those of guanine (G) moieties are covered by an intensive current of hydrogen evolution. Because cyclic voltammetry allows us to monitor only the oxidation processes of the G reduced species, the effect of hydrogen evolution reaction (HER) was studied by means of these anodic signals. For different vertex potentials (from -1.60V to -1.85V) and a different number of cycles (1-5), all heptamers in buffered solutions (pH5.8) were tested and double-peak G oxidation signals (GI and GII) evaluated. The different structures of the heptamers in solutions are confirmed by NMR spectra. Both the impact of hydrogen evolution into the production of 7,8-dihydroguanine moieties forming together with hydrogen in negative potentials and the catalytic effect of heptamers on hydrogen evolution are discussed.

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