Publication details

Recent progress in electrochemical sensors and assays for DNA damage and repair


FOJTA Miroslav DANHEL Ales HAVRAN Ludek VYSKOCIL Vlastimil

Year of publication 2016
Type Article in Periodical
MU Faculty or unit

Central European Institute of Technology

Field Analytic chemistry
Keywords DNA damage; DNA modification; DNA repair; Base mismatch; Electrochemical detection; Biosensor; Bioassay; Enzyme; DNA cleavage; DNA drug interactions
Description DNA in cells is permanently exposed to endogenous or exogenous chemical or physical agents which may cause chemical changes in the DNA structure collectively called "DNA damage", which may have severe impacts on human health. Therefore, efficient techniques to detect DNA damage are sought, among which electroanalytical methods hold an important position. This review is focused on recent progress in the field of electrochemical sensors and assays for DNA damage, particularly on typical applications of advanced electrochemical techniques in detecting oxidative DNA damage, abasic lesions, base mismatches, non-covalent interactions with drugs and other substances. Special attention is paid to combinations of electrochemical detection with biochemical tools such as enzymatic systems mimicking metabolic activation of xenobiotics, or DNA repair enzymes converting specific nucleobase lesions to species offering more facile electrochemical detection. These applications create conditions for application of electroanalytical approaches in biological research, including studies of DNA repair processes. (C) 2015 Elsevier B.V. All rights reserved.
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