Publication details
Vliv subletálních koncentrací dezinfekčních látek na tvorbu biofilmu v prostředí masného průmyslu
| Title in English | Effect of sub-lethal concentrations of disinfectants on biofilm formation in the environment of the meat industry |
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| Authors | |
| Year of publication | 2016 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | In the meat industry, biofilm is a persistent source of contamination of microorganisms. These microorganisms come into contact with food and can cause food spoilage, as well as the transmission of pathogenic microorganisms involved in foodborne illnesses. Pathogenic microorganisms having the ability to form a biofilm include Staphylococcus aureus, pathogenic strains of Escherichia coli, Listeria monocytogenes, Salmonella spp., Yersinia enterocolitica or Campylobacter jejuni. The biofilm consists primarily of microbial cells and extracellular polymeric material (EPS) which protects these cells against the action of disinfectants. Their penetration into the biofilm is thus reduced and at the same time EPS makes it more difficult to wash biofilm disinfectant residues. Remnants of disinfectants accumulate and persist in biofilm environments at low concentrations to which microorganisms can adapt. In addition, based on previous studies, it can be assumed that just sub-lethal concentrations of disinfectants can induce biofilm formation. The aim of this pilot study was to test the influence of sublethal concentrations of three currently used in the food industry on disinfectants used to induce biofilm formation in E. coli and S. aureus strains isolated from meat establishments. The following disinfectants have been tested: benzalkonium chloride, ethanol and peroxyacetic acid. The induction of biofilm formation was monitored by measuring the fluorescence signal after staining with SYTO9. The highest induction of biofilm formation in S. aureus was observed in culture with 4x10-4% BAC; 1.25% ethanol and 3x10-5% peroxyacetic acid; for E. coli cultures with 2x10-8% BAC; 5% ethanol and 3x10-6% peroxyacetic acid. These measurements confirm that sub-lethal concentrations of selected disinfectants can induce biofilm formation in E. coli and S. aureus. |