Publication details
Využití real-time PCR pro detekci Yersinia enterocolitica ve vzorcích rostlinného původu
| Title in English | Using real-time PCR for detection of Yersinia enterocolitica in samples of vegetable origin |
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| Authors | |
| Year of publication | 2015 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Introduction Genus Yersinia includes about 11 non-pathogenic and pathogenic species including Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. These three bacteria are facultative intracellular pathogens characterized by a number of virulence factors. Yersinia enterocolitica is the cause of food infections transmitted via the faecal-oral route. Especially for young children and preschoolers, the occurrence of yersiniosis is characterized by acute enteritis, which is accompanied by fever and diarrhea. The aim of this study was to find out the occurrence of yersinias using molecular biology methods on the surface of foods of plant origin intended for direct consumption. This commodity may be risky from the point of view of food microbiological safety. Material and methods In this study, samples of sliced and frozen vegetables (197) and small fruit (141) from the market were examined. Using optimized procedures involving rinsing, homogenization and centrifugation were yielded pellets containing bacterial contamination present on the surface of the processed sample (100 g of rinse sample). The DNA from this pellet was isolated using a Power soil DNA isolation kit (MoBio, USA) according to a manufacturer's procedure slightly modified for mechanical homogenization in a Magnalyser using 0.1 mm zirconia beads. The actual detection of pathogenic Y. enterocolitica was performed using a species-specific qPCR targeting the ompF gene (occurrence in all Yersinia species) and the ail gene (the virulence factor of the pathogenic Y. enterocolitica). Results A total of 338 samples of vegetables and small fruits were used to evaluate the occurrence of Y. enterocolitica in the samples. Of these, 43 samples (12.7%) were positive for the presence of bacteria of genus Yersinia. Twenty-four samples (7%) were positive for the presence of Yersinia spp. and in nineteen samples (5.6%) pathogenic Yersinia enterocolitica was detected using qPCR. |