Publication details

Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

Authors

REBROŠOVÁ Katarína SILER M. SAMEK O. RŮŽIČKA Filip BERNATOVA S. JEZEK J. ZEMANEK P. HOLÁ Veronika

Year of publication 2017
Type Article in Periodical
Magazine / Source FUTURE MICROBIOLOGY
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.2217/fmb-2016-0224
Field Microbiology, virology
Keywords Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus
Description Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
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