Publication details

Sensitive determination of malondialdehyde in exhaled breath condensate and biological fluids by capillary electrophoresis with laser induced fluorescence detection


LAČNÁ Júlia FORET František KUBÁŇ Petr

Year of publication 2017
Type Article in Periodical
Magazine / Source Talanta
MU Faculty or unit

Central European Institute of Technology

Field Analytic chemistry
Keywords Malondialdehyde; Capillary electrophoresis; Laser induced fluorescence; Exhaled breath condensate; Blood plasma; Saliva
Description In this work, a sensitive capillary electrophoresis method with laser induced fluorescence detection for determination of malondialdehyde in various biological fluids was developed. Malondialdehyde reacts with thiobarbituric acid under optimized conditions of pH=2, reaction time of 60 min and temperature of 90 degrees C, yielding an adduct that can be separated in a 50 mM sodium borate background electrolyte at pH 9. The separation of the formed adduct was accomplished in less than 6 min with limit of detection of 1.1 nM due to the use of 532 nm laser module, exactly matching the maximum excitation wavelength of the formed adduct. The developed method offers unprecedented sensitivity and was for the first time used for analysis of malondialdehyde in exhaled breath condensate. The method proved to be also applicable to other samples of biological fluids, such as blood plasma and saliva.
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