Publication details

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

Authors

UHLÍKOVÁ Hana SOLANSKÝ Martin HRDINOVÁ Vendula ŠEDO Ondrej KAŠPAROVSKÝ Tomáš HEJÁTKO Jan LOCHMAN Jan

Year of publication 2017
Type Article in Periodical
Magazine / Source Journal of Plant Physiology
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.jplph.2016.12.005
Keywords Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase
Description Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.
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