Publication details

Acetogenic microorganisms in operating biogas plants depending on substrate combinations



Year of publication 2019
Type Article in Periodical
Magazine / Source Biologia
MU Faculty or unit

Faculty of Science

Web Full Text
Keywords Acetogenic microorganisms; Biogas Anaerobic digesters; Methane; Renewable energy
Description In recent years, various substrates for increasing the production of biomethane are increasingly exploring. The effect of such substrates on methanogenesis is investigated mainly in small volume digesters. However, operating acetogenic biogas plants depending on substrates combinations and monitoring of microbial communities in the anaerobic digesters located in the Czech Republic have never been studied yet. The variation in the distribution of main acetogenic genera in eight biogas plants based on different input substrates ratio was studied. The genera composition was identified by sequencing of the 16S rRNA gene. In total, 33 genera of this group of microorganisms were found out. The highest percentage (30.2%) of acetogens compared to other biogas plants was observed in Modřice biogas plant with the input substrate biological sludge and primary sludge (50/50, w/w %). The smallest percentage (1.14%) and variation in the distribution of acetogenic microorganisms was found in Čejč biogas plant, with the input substrate maize silage and liquid pig manure (80/20, w/w %). For the biogas plant operator it would be important to know the composition of a consortium of anaerobic microorganisms that are present in anaerobic digesters (in two regions in the Czech Republic) processing different input combinations of a raw material. This paper characterizes the effect of the input raw materials on the variation in the distribution of the microbial community.
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