Publication details
Diagnostický prostředek pro simultánní detekci humánních norovirů (NoV GI a NoV GII), viru hepatitidy A, viru hepatitidy E (HEV-1, HEV-3), adenovirů 40/41 a rotavirů A
| Title in English | Diagnostic tool for simultaneous detection of human noroviruses (NoV GI and NoV GII), hepatitis A virus, hepatitis E virus (HEV-1, HEV-3), adenovirus 40/41 and rotavirus A |
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| Authors | |
| Year of publication | 2019 |
| MU Faculty or unit | |
| Description | Because of its sensitivity, specificity and possibility of quantification, the most suitable method of detection of viruses in food and environment is a real-time polymerase chain reaction (qPCR). In the case of viruses with RNA genome, qPCR is preceded by a reverse transcription step. However, this approach has a major disadvantage in a limited ability to create multiplex systems for rapid detection of multiple pathogens. Therefore, the diagnostic tool which allows in vitro detection of NoV (NoV GI, NoV GII), HAV, HEV (HEV-1, HEV-3), AdV 40/41 and RVA in various types of matrices (water, food, clinical material, swabs of environment or hands) in one reaction has been developed. This detection tool, “xMAP panel - detection of NoV (NoV GI, NoV GII), HAV, HEV (HEV-1, HEV-3), AdV 40/41 and RVA”), is unique in its composition and ease of use. The kit also includes an external sample analysis control (EAC) that allows valid analysis of each single sample |