Publication details

Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge

Authors

SUMAN Jachym ZUBROVA Andrea ROJIKOVA Katerina PECHAR Radko ŠVEC Pavel CAJTHAML Tomas ULBRICH Pavel RIDL Jakub STRNAD Hynek UHLIK Ondrej

Year of publication 2019
Type Article in Periodical
Magazine / Source International Journal of systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003493#tab2
Doi http://dx.doi.org/10.1099/ijsem.0.003493
Keywords Rhodobacteraceae; Pseudogemmobacter bohemicus; 16S rRNA gene analysis; chemotaxonomic characterization; percentage of conserved proteins; heavy metals
Description The creamy white to beige, aerobic, non-motile, ovoid to rod-shaped, Gram-stain-negative strain, Cd-10(T), was isolated from heavy-metal-contaminated sludge from a decantation basin of a heavy metal processing factory based on its ability to tolerate CdCl2 in the cultivation medium. In the reconstruction of its phylogeny based on 16S rRNA gene sequences, strain Cd-10(T) clustered with species of the genera Gemmobacter, Xinfangfangia, Tabrizicola and Rhodobacter within the family Rhodobacteraceae. Its 16S rRNA gene sequence exhibited 96.32% pairwise similarity to the type strain of Xinfangfangia soli, 95.3 % to that of Gemmobacter intermedius, followed by Tabrizicola fusiformis (95.10 %), Rhodobacter sediminis (94.88 %), Gemmobacter nectariphilus and Rhodobacter capsulatus (both 94.81%). The major respiratory quinone was Q-10 accompanied by Q-9, the fatty acid profile consisted predominantly of C-18:1 omega 7C, C-18:0, C-16:0 and C-16:1 omega 7c, the major polar lipids were phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and diphosphatidylglycerol. An analysis of the percentage of conserved proteins deduced from draft or complete genomic sequences of strain Cd-10(T) and representatives of its closest relatives suggested that strain Cd-10(T) is a member of a novel genus within the Rhodobacteraceae family for which we propose the name Pseudogemmobacter. Strain Cd-10(T) (=DSM 103618(T)=NCCB 100645(T)) is the type strain of Pseudogemmobacter bohemicus gen. nov., sp. nov., the type species of the genus Pseudogemmobacter gen. nov.

You are running an old browser version. We recommend updating your browser to its latest version.

More info